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| Haag,Karen Luisa. |
| Abstract Symbioses are ubiquitous and have played an influential role in the evolution of life on Earth. Genomic studies are now revealing a huge diversity of associations among hosts and their microbiotas, allowing us to characterize their complex ecological and evolutionary dynamics. The different transmission modes and the asynchronous cell proliferation of the numerous symbionts associated with one host generate a genomic conflict ought to be solved. Two disputing views have been used to model and predict the outcome of such conflicts. The traditional view is based on community ecology, and considers that selection at the level of individuals is sufficient to explain longstanding associations among species. A new perspective considers that the host and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genomics; Metagenomics; Microbiota; Symbiosis; Holobiont. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200189 |
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| Clerissi, Camille; De Lorgeril, Julien; Petton, Bruno; Lucasson, Aude; Escoubas, Jean Michel; Gueguen, Yannick; Dégremont, Lionel; Mitta, Guillaume; Toulza, Eve. |
| Pacific Oyster Mortality Syndrome (POMS) affects Crassostrea gigas oysters worldwide and causes important economic losses. Disease dynamic was recently deciphered and revealed a multiple and progressive infection caused by the Ostreid herpesvirus OsHV-1 μVar, triggering an immunosuppression followed by microbiota destabilization and bacteraemia by opportunistic bacterial pathogens. However, it remains unknown if microbiota might participate to protect oysters against POMS, and if microbiota characteristics might be predictive of oyster mortalities. To tackle this issue, we transferred full-sib progenies of resistant and susceptible oyster families from hatchery to the field during a period in favor of POMS. After 5 days of transplantation, oysters from... |
| Tipo: Text |
Palavras-chave: Holobiont; Microbiome; Metabarcoding; Fitness; Bacteria. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00621/73332/72500.pdf |
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| Cambon-bonavita, Marie-anne; Aubé, Johanne; Cueff-gauchard, Valerie; Reveillaud, Julie. |
| Background Free-living and symbiotic chemosynthetic microbial communities support primary production and higher trophic levels in deep-sea hydrothermal vents. The shrimp Rimicaris exoculata, which dominates animal communities along the Mid-Atlantic Ridge, houses a complex bacterial community in its enlarged cephalothorax. The dominant bacteria present are from the taxonomic groups Campylobacteria, Desulfobulbia (formerly Deltaproteobacteria), Alphaproteobacteria, Gammaproteobacteria, and some recently discovered iron oxyhydroxide-coated Zetaproteobacteria. This epibiotic consortium uses iron, sulfide, methane, and hydrogen as energy sources. Here, we generated shotgun metagenomes from Rimicaris exoculata cephalothoracic epibiotic communities to reconstruct... |
| Tipo: Text |
Palavras-chave: Niche partitioning; Rimicaris; Metagenome-assembled genomes; Zetaproteobacteria; Holobiont; Hydrothermal vents. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00689/80089/83128.pdf |
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| Clerissi, Camille; Brunet, Sebastien; Vidal-dupiol, Jeremie; Adjeroud, Mehdi; Lepage, Pierre; Guillou, Laure; Escoubas, Jean Michel; Toulza, Eve. |
| Previous observations suggested that microbial communities contribute to coral health and the ecological resilience of coral reefs. However, most studies of coral microbiology focused on prokaryotes and the endosymbiotic algae Symbiodinium. In contrast, knowledge concerning diversity of other protists is still lacking, possibly due to methodological constraints. As most eukaryotic DNA in coral samples was derived from hosts, protist diversity was missed in metagenome analyses. To tackle this issue, we designed blocking primers for Scleractinia sequences amplified with two primer sets that targeted variable loops of the 18S rRNA gene (18SV1V2 and 18SV4). These blocking primers were used on environmental colonies of Pocillopora damicornis sensu lato from two... |
| Tipo: Text |
Palavras-chave: Holobiont; Protists; Symbiosis; Metabarcoding; Blocking primer; Scleractinia; Pocillopora damicornis. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00457/56823/58661.pdf |
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| Gobet, Angelique; Mest, Laetitia; Perennou, Morgan; Dittami, Simon M.; Caralp, Claire; Coulombet, Celine; Huchette, Sylvain; Roussel, Sabine; Michel, Gurvan; Leblanc, Catherine. |
| Background: Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host. In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized. Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass. Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet. We investigated the effect of four... |
| Tipo: Text |
Palavras-chave: Microbe-host interactions; Digestive microbiota; Abalone; Macroalgae; Holobiont. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00489/60071/63368.pdf |
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| Clerissi, Camille; Guillou, Laure; Escoubas, Jean Michel; Toulza, Eve. |
| Background Microbiome of macroorganisms might directly or indirectly influence host development and homeostasis. Many studies focused on the diversity and distribution of prokaryotes within these assemblages, but the eukaryotic microbial compartment remains underexplored so far. Results To tackle this issue, we compared blocking and excluding primers to analyze microeukaryotic communities associated with Crassostrea gigas oysters. High-throughput sequencing of 18S rRNA genes variable loops revealed that excluding primers performed better by not amplifying oyster DNA, whereas the blocking primer did not totally prevent host contaminations. However, blocking and excluding primers showed similar pattern of alpha and beta diversities when protist communities... |
| Tipo: Text |
Palavras-chave: Holobiont; Metabarcoding; Microbiome; Ostreoida; Crassostrea. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00636/74799/75165.pdf |
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| Nguyen-kim, Hanh; Bouvier, Thierry; Bouvier, Corinne; Ngoc Bui, Van; Le-lan, Huong; Bettarel, Yvan. |
| The periodic rise in seawater temperature is one of the main environmental determinants of coral bleaching. However, the direct incidence of these episodic thermal anomalies on coral-associated microbiota and their subsequent effects on coral health are still not completely understood. In this study, we investigated the dynamics of three main microbial communities of the coral holobiont (e.g., Symbiodinium, bacteria and viruses), during an experimental thermal stress (+4 degrees C) conducted on the scleractinian Fungia repanda. The heat-treatment induced coral bleaching after 11 days and resulted in a final elevation of ca. 9, 130 and 250-fold in the abundance of mucosal viruses, bacteria, and Symbiodinium, respectively. On the contrary, the proportion of... |
| Tipo: Text |
Palavras-chave: Coral bleaching; Thermal stress; Bacteria; Viruses; Holobiont; Vietnam. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00626/73823/73642.pdf |
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